Hello all,

I am a biologist biotechnologist with both degrees. I have focused alot on microbiology and cell culture.

I am fascinated with slime molds and true fungi. And I have edited several fungi and protists to produce new colors, make cellulase variants, be bioluminescent ect.

Now ive tried the major and og methods of editing microbes with

Physarum Polycephalum or yellow slime mold.

And tried with tubnifera magna (but this is couldnt find a media to keep it alive, was younger less experienced. still upset as ive looked for another sample for years and never found it.)

But the slime mold never holds onto the new genes or they don't incorporate properly. Ive gotten Physarum Polycephalum to hold onto a plasmid used to make bacteria glow in bio classes. but within a day or 3. it is completely purged.

Now I have been thinking that perhaps I can get it to hold onto plasmids through a different brute force.

I am going to search for some more geenticly acceptable genes for a slime mold as i imagine the bacterial genes don't translate perfectly.

but in the mean time. I have recently forced a culture of ecoli, and 2 cultures of as of yet unidentified bacteria that I found in a contaminated slime mold plate and which I watched my Nesse eat under microscope.

so now I have 3 bacteria that nesee will eat. and all 3 have been forced to take in multiple copies of bioluminescent plasmids.

and now im going to be feeding nesse these cultures daily to see if over time the slime mold will start recognizing the plasmids as just another piece of dna in its single cell beauty.

not super hopeful. but sometimes you just need time. and if nesse doesn't start producing waves of bioluminescs as it pulses after a month. i will give up.

Until I get my next idea.

anyone got any advice?

Mehedeby, Sweden.

I was photographing this raspberry slime mold and in the photo I noticed this tiny white dot on a stem. It resembles Comatricha nigra young fruiting body, but it is much smaller than 1 mm.
Europe

9ish inches wide, not very thick at all. North Cascades, WA.

This thing is infesting my flowerbeds! Takes all the wood chips and clumps them together. This mulch is from last fall when i replaced this same flowerbed because of this mold or fungi taking over. Not sure what to do or what i can use instead of wood chips. Thumb of Michigan 6a. This flowerbed gets part to very little sun and stays moist but not too wet. Funny enough the area around my hose which is always more wet is unaffected and the wood chips are fine to turn over there.

I bought some of this online and now need help to ID this guy, names jerry. (its apparently sterile, i have not opened it and looks like the dog vomit mold however thats more of a blob and not all veins like, which is like the one I have)

Over the past few months, I’ve written this comprehensive guide to slime mold cultivation and observation. It’s essentially a review, plus some experiences of my own and others, and it should be suitable for both general enthusiasts and professionals. However, I don’t have time to translate it into other languages for now, so I’m posting the PDF and editable DOCX files here. You can read it with translators or similar tools, but I hope someone will share a translated version! Also, if you have any revision suggestions, questions, or text/images you’d like to add, feel free to leave a comment—I may include them in the next update! I hope it’s useful to you, and you’re welcome to freely share it on other platforms! Also, a tribute to ian!

Kentucky, United States

First is under bathroom subfloor.

Second is under the edge of a step.

I finally found slime mold in the wild on a walk today!! To say I was excited is an understatement 😂 my mom had to listen to the spiel about it cause she was with me. I wanted to harvest and start to cultivate but I think I'd probably kill it lol

Shot with OM-5 and Zuiko 60mm f2.8 macro

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